SinoXivA Probiotic Formula Reshapes Oral-Gut Microbiota, Attenuates the Gut Resistome, and Supports Lean Growth in Preschoolers: A Multi-Omics Trial
Abstract
The preschool years represent a modifiable window for shaping growth and microbiome development. In a two-month cluster randomized trial of 317 Chinese kindergarten children, we evaluated a formula enriched with Bifidobacterium animalis subsp. lactis Probio-M8. The intervention promoted greater gains in height and fat-free mass without increasing body mass index. Multi-omics profiling (saliva 2bRAD-M, fecal metagenomics, metabolomics) revealed site-specific microbiome remodeling, reduced oral-gut microbial coupling, and a net contraction of the gut resistome, particularly in efflux-associated genes. The fecal metabolome showed a biphasic response: early shifts in energy metabolism gave way to later enrichment of lipid mediators (e.g., resolvin D2) and neuroactive compounds. Integrative networks linked Bifidobacterium-enriched communities and metabolite signatures to improved growth and lower antibiotic use. These findings demonstrate that probiotic nutrition during preschool can coordinately enhance lean growth, refine microbiome organization, and reduce antimicrobial resistance potential in real-world settings.
Keywords
Citation Information
@article{mengzhang2026,
title={A Probiotic Formula Reshapes Oral-Gut Microbiota, Attenuates the Gut Resistome, and Supports Lean Growth in Preschoolers: A Multi-Omics Trial},
author={Meng Zhang and Xin Han and Zhengdong Liu and Kaiyang Liu and Xuezhen Zhou and Xingwang Ye and Zhenyu Yang and Yanmei Ma and Lai-Yu Kwok and Yifan Duan and Wenyi Zhang and Heping Zhang},
journal={Research Square},
year={2026},
doi={https://doi.org/10.21203/rs.3.rs-9483549/v1}
}
