Objective This study aimed to evaluate serum Omentin Levels and its Association with Glycemic Control, Lipid Profile, and Obesity in Palestinian Females with Type 2 Diabetes Mellitus.Methods This observational case–control study was recruited 200 participants aged (30–60 years) divided into a control group and diabetic patients categorized according to their BMI as normal weight group (BMI < 25 kg/m2), overweight group (BMI 25 -29.9 kg/m2), and obese group (BMI ≥ 30 kg/m2) with 50 participants in each study groups. Sociodemographic data, clinical history, anthropometric measurements, blood pressure, and pulse rate were collected from all subjects. The levels of biochemical parameters; omentin, insulin, glycated hemoglobin, fasting glucose, and lipid profile were measured and statistically analyzed using one-way ANOVA and correlation analysis.Results Compared with controls, diabetic participants showed significantly increased body weight, waist circumference, BMI, and both systolic and diastolic blood pressure (P < 0.05). Serum omentin level was significantly increased in overweight diabetic and obese diabetic patients compared to control (P < 0.05). HbA1c and fasting glucose levels were significantly higher across all diabetic groups (P < 0.001). All diabetic patients had increased cholesterol and triglyceride levels with decreased HDL values indicating dyslipidemia. Persons Correlation analysis showed significant correlations between BMI and anthropometric measures. Omentin levels were significantly correlated with lipid markers.Conclusions The findings suggest that serum omentin is associated with metabolic disturbances in T2DM patients. It can be used as a promising biomarker for T2DM patients and has relation with obesity and dyslipidaemia.