Fungi of the genus Fusarium cause damage in different types of crops such as tomatoes, onions, bananas, among others, causing different diseases such as wilt disease or Fusarium Head Blight (FHB). Recent studies show that Fusarium graminearum has a high resistance to fungicide and alternative treatment needs to be searched. There are different ways to treat diseases caused by Fusarium, between them, the most relevant is the use of biological control agents. The use of actinobacteria as a biological control agent is considered promising, due to the fact that the most important and known characteristic of this bacteria is the production of secondary metabolites and bioactive compounds. In the present study, eleven actinobacteria isolates were identified by morphological characteristics and submitted to antifungal assay against two isolates of Fusarium graminearum. As a result of the preliminary identification one isolate was identified as Nocardia spp., seven isolates as Streptomyces spp. and three remained unidentified morphologically. The antagonistic assay was performed using the plate confrontation method, against the two strains of Fusarium graminearum. The isolate LMAF, previously identified as Streptomyces avidinii, showed activity against both Fusarium graminearum isolates. The relation between the pathogen and the Streptomyces avidinii LMAF remain unsure, due to the fact that it can be direct, where the isolate is producing substances that control the growth, or indirect, where both are competing for nutrients and space. Both cases lead to the potential use of isolate LMAF as a biological control agent.