Purpose: To assess prostate biopsy uptake and its predictors among patients previously found to have elevated Prostate-Specific Antigen results at the Kyabirwa Surgical Center. Method: An analytical cross-sectional design was employed to study male patients with elevated PSA levels (> 10 ng/mL) at the Kyabirwa Surgical Center. They were consecutively sampled, located in their areas of residence, and engaged in structured interviews. Data were collected and analyzed descriptively using SPSS version 26, with a log-binomial model for relationship analysis. Results: The proportion of patients with elevated PSA levels at the Kyabirwa Surgical Center (KSC) who underwent prostate biopsy was 55.8%, the majority of whom underwent biopsy at the center (72.4%). Perceived good health and the ability to perform biopsy at any time lowered the prevalence of uptake.However, educating patients about prostate health, the cost of a prostate biopsy, the mean PSA results, the importance of a biopsy, how it is performed by healthcare providers at KSC, and being followed up by healthcare workers at KSC to determine compliance with the instructions to perform a biopsy increases biopsy uptake. Conclusion: The uptake of prostate biopsy among patients previously found to have elevated Prostate-Specific Antigen levels at the Kyabirwa Surgical Center is fair; only half of them go ahead to undergo biopsy. Antagonists of prostate biopsy uptake are patient-related, while all protagonists are institutional and specifically related to post-test patient education.